Exonuclease III is a 3'-5' exonuclease releasing 5' mononucleotides from dsDNA and exhibits AP-specific endonuclease, 3' phosphatase and RNAse H activities. Source: E.coli cells carrying a cloned E.coli xth gene. Storage Buffer: Supplied as a liquid in 50mM Tris-HCl, pH 8.0, 50mM KCl, 1mM DTT, 50% glycerol. Reaction Buffer (10X) E9008-05: Supplied as a liquid in 660mM Tris-HCl, pH 8.0, 6.6mM MgCl2 Unit Definition: One unit of Exonuclease III catalyzes the release of 1nmol of acid soluble reaction products from E. coli [3H]-DNA in 30 min at 37°C. Applications: Suitable for use in construction of nested unidirectional deletions of DNA fragments, generation of single-stranded template for DNA dideoxy sequencing, site-directed mutagenesis, cloning of PCR products and preparation of strand-specific probes.