1321N1 is a human astrocytoma cell line isolated in 1972 as a sub clone of the cell line 1181N1 which in turn was isolated from the parent line U-118 MG (one of a number of cell lines derived from malignant gliomas by J Ponten (Ponten, J., Macintyre, E. H. (1968) PMID: 4313504).1321N1 has been shown to have very similar STR profile data to U-118 MG.