The blood samples are enzymatically pre-treated and then transferred in the wells of a microtiter plate[PLATE] coated with Lactobacillusfermentum.The addition of vitamin B1 in either standards [STD],control [CTRL1,CTRL2] or samples gives a vitamin B1-dependent growth response until vitamin B1 is consumed.After incubation at 37 °C for 48 hours, the growth of Lactobacillus fermentum is measured turbidimetrically at 610 - 630 nm (alternatively at 540 - 550 nm) in an ELISA-reader and a standard curve is generated from the dilution series.The amount of vitamin B1 is directly proportional to the turbidity. Sample Types: whole blood. Range: 3 - 15 μg/L. Sensitivity: 3 μg/L. Time: 24 hours ? The Eagle Biosciences Vitamin B1 is a microtiter plate test kit based on a microbiological assay which measures the vitamin B1 content in serum. The test kit contains all required reagents, e.g. standard, medium and microtiter plate coated with a specific microorganism, sufficient for 96 determinations including standard curves. An ELISA reader is required for evaluation of the vitamin B1 content. For use in human and veterinary medicine and in research. The Biotin Assay kit is For Research Use Only.