Glycosphingolipids (GSLs) are ubiquitous components of eukaryotic cell membranes. GSLs are synthesized by sequential addition of saccharides to the hydroxyl group at the C-1 position of the ceramide (Cer) backbone. GSLs are mainly located in the extracellular leaflet of the plasma membrane, where they are involved in several functions such as cell-to-cell interaction and recognition.