The sVCAM-1 Kit is a solid phase sandwich Enzyme Linked Immuno-Sorbent Assay (ELISA). A monoclonal antibody specific for sVCAM-1 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known sVCAM-1 concentrations and unknowns are pipetted into these wells. During the first incubation, the sVCAM-1 Antigens and a biotinylated monoclonal antibody specific for sVCAM-1 are simultaneously incubated. Sample Types: serum, plasma, cell culture supernatant. Range: 1.56-50 ng/ml. Sensitivity: 0.6 ng/ml. Time: 1h40 ? The Eagle Biosciences Human sVCAM-1/CD106 ELISA is a solid phase sandwich ELISA for the in-vitro qualitative and quantitative determination of soluble Vascular Cellular Adhesion Molecule-1 (sVCAM-1) in cell culture supernatants, buffered solutions or human serum, plasma, or other body fluids. This assay will recognize both natural and recombinant human sVCAM-1. The Eagle Biosciences Human sVCAM-1/CD106 ELISA Assay kit is for Research Use Only.