RAT IgG2a isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein G. In an Ouchterlony double diffusion assay the material is non-reactive with antisera to rat IgG1, IgG2b, IgG3, IgM, and IgA. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rat IgG and anti-Rat serum. Light and heavy chain composition has been confirmed.
Size
500 μg
Concentration
0.5 mg/mL
Applications
ELISA, FLISA, Flow Cytometry, IF Microscopy. RAT IgG2a isotype control can be utilized as a control or standard reagent in Western Blotting, Flow Cytometry, and ELISA experiments where determination of sample isotype is important. Specific conditions should be optimized by user.
Other Names
Rat Isotype Control, Rat IgG2a PE isotype, RAT IgG2a subclass isotype control