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Description
Magic? Rabbit anti-Human CD3 (aa 156-168) polyclonal antibody for IHC. CD3 consists of atleast four different components (β,δ,ε,ζ) of 20-28 kDa. On thelymphocyte cell surface, CD3 is non-covalently associated with the T-cellreceptor (TCR). It is believed that the CD3 components of the TCR/CD3 complexmediate signal transduction upon antigen recognition by TCR. CD3 is expressedby T cells in thymus, bone marrow, peripheral lymphoid tissue and blood. Theonly other normal cell type known to be labelled by antibodies to CD3, isPurkinje cells in the cerebellum. The CD3 antigen is first detectable inearly thymocytes and its appearance probably represents one of the earliestsigns of commitment to the T-cell lineage. In immature thymocytes the CD3expression is solely cytoplasmic, membrane-associated CD3 appearssubsequently upon cell maturation. The majority of T-cell neoplasms expressthe CD3 antigen, while it is absent from non-T-cell lymphoid malignancies.Consistent with the pattern of synthesis of the antigen in normal thymocytes,the earliest site where CD3 is detectable within neoplastic cells is in thecell cytoplasm. CD3 has been deemed an essential marker for the initialevaluation of chroniclymphoproliferative disorders. Large granular lymphocyteleukaemia of T-cell type (T-LGL) is characterized by the CD3+/CD57+/CD56-immunophenotype and clonal rearrangement of the T-cell receptor genes.
Immunogen
Syntheticpeptide comprising amino acids 156-168 from the cytoplasmic part of the humanCD3ε?chain coupled to thyroglobulin.