Sequence: 5'-A G C^G C T-3' 3'-T C G^C G A-5' Source: Escherichia coli RFL47 Concentration: ~10u/ul Unit Definition: One unit is defined as the amount of Eco47III required to digest 1ug of lambda DNA-Eco81I fragments in 1 hr at 37°C in 50ul assay buffer. Storage and Diluent Buffer: Supplied as a liquid in 10mM Tris-HCl, pH 7.4, 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA, 50% glycerol. Supplied with: R1625: Supplied as a liquid in 33mM Tris-acetate, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA, pH 7.9 at 37°C. R1625-03: Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 10mM MgCl2, 100mM sodium chloride, 0.1mg/ml BSA. Incubate at 37°C Double Digests: R1625 is provided to simplify buffer selection for double digests. 98% of Eco47III, works well in a 1x or 2x.