5'-C^G G C C G-3' 3'-G C C G G^C-5' Sequence: 5'-C^G G C C G-3' 3'-G C C G G^C-5' Source: Escherichia coli RFL52 Concentration: 10u/ul Unit Definition: One unit is defined as the amount of Eco52I required to digest 1ug of lambda DNA-Eco81I fragments in 1 hour at 37°C in 50ul of recommended reaction buffer.