Trueblot? Magnetic Beads are uniform, non-aggregating, super-paramagnetic beads consisting of a ferric oxide core functionalized with various silane groups. The super-paramagnetic nanoparticles are coupled with a biomolecule, such as Biotin, and are specifically designed, tested and quality controlled for magnetic separation, isolation and purification of avidin and streptavidin-labeled components using manual or automatic platforms. Biotin magnetic beads are stable, pre-blocked beads with high binding capacity that provide rapid and efficient biomolecule purification from complex samples. Bead mean diameter is ~0.5 μm, bead concentration is 5 mg/mL, and binding capacity is ≥ 30 μg streptavidin/mg of beads.
Size
2 mL
Concentration
5.0mg/mL
Applications
Immunoprecipitation, Western Blot. Trueblot? Biotin magnetic beads can be used in immunoassays or molecular diagnostics when avidin, streptavidin is bound to proteins or oligonucleotides. Biotin magnetic beads can also be used in nucleic acid isolation, protein purification, and cell separations. Biotin magnetic particles are incubated with the avidin or streptavidin labeled solution and then separated by magnets. After the unbound particulates are washed from the particles, the bound avidin are eluted from the particles using the elution buffer. The particles are then magnetically separated from the eluted solution, which is removed manually.
Other Names
Magnetic beads, particles, microparticles, paramagnetic nanoparticles, magnets, protein antibody, DNA, biotinylated avidin, neutravidin, isolation, purification