TrueBlot? Immunoprecipitation and Western Blot Kit for DYKDDDDK (FLAG?) Epitope Tag
Description
This TrueBlot? Immunoprecipitation and Western Blot Kit for DYKDDDDK (FLAG?) Epitope Tag allows for the detection of FLAG? -tagged recombinant protein present in cell lysates provided by the user.Mouse TrueBlot? ULTRA Antibody Peroxidase Conjugate was prepared from tissue culture supernatant by Protein G affinity chromatography. Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum. Reactivity is observed against native Mouse IgG by both Western blot and ELISA.
Size
1 kit
Concentration
n/a
Applications
Immunoprecipitation, Western Blot. Mouse IgG TrueBlot? ULTRA is provided as 1000X solution. In order to conserve reagent, we recommend incubating the blots from minigels in sealed bags (removing as much air as possible) with minimal volume (2-3 mls).Mouse IgG TrueBlot? ULTRA. An HRP-conjugated second step reagent reacting with mouse IgGs for optimal signal detection in immunoprecipitation/immunoblotting experiments.Note that there are three key procedural considerations:1. Protein A or G beads may be used with the mouse, goat and sheep TrueBlot secondaries but not with the rabbit TrueBlot secondary. Use of protein A or G beads with the rabbit TrueBlot will result in contaminating bands.2. Immunoprecipitate should be completely reduced.3. Milk should be used as the blocking protein for the immunoblot. The TrueBlot? Immunoprecipitation and Western Blot Kit for DYKDDDDK (FLAG?) Epitope Tag components are sufficient for 20-25 miniblots.Components:1. Mouse IgG TrueBlot ULTRA: 50 μL 18-8817-31 2. TrueBlot Enhancer Solution: 25 mL3. TrueBlot Blocker: 10 g4. TrueBlot Assay Buffer: 30 mL 20X5. TrueBlot Substrate A: 12.5 mL6. TrueBlot Substrate B: 12.5 mL7. Anti-Mouse Ig IP Beads: 2.5 mL Binds 0.4 mg Ig/mL beads8. Antibody for the detection of FLAG? conjugated proteins (MOUSE) Monoclonal Antibody: 100 μg9. Western Blot Incubation TraySpecial Notes:Upon initial use of the IP beads, we recommend that the vial be inverted several times to get the beads into suspension. We recommend to use a large bore pipet to pipet up the liquid for use.For storage of the opened vial of beads, we recommend that the vial cap be sealed with parafilm to help prevent evaporation of the buffer.
Other Names
HRP, TrueBlot, HRP TrueBlot ULTRA, Peroxidase TrueBlot, TrueBlot for IP/WB, TrueBlot for immunoprecipitation, TrueBlot for western blotting, Flag Western Blot, Western Blotting Kit, Chemiluminescent Flag Kit, Immunoblotting Kit, FLAG?, HRP Anti FLAG, DDK, FLAG, FLAG antibody, anti-Flag, anti-DDK, DDK antibody