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Description
Anti-mCherry Nanobody Affinity Gel. mCherry is a red fluorescent protein that is often used to tag target proteins and to monitor their subcellular localization. It is the most widely used photostable protein in the class of red-shifted fluorescent proteins. mCherry derives from proteins initially isolated from Cnidarians.Nanobodies are heavy chain only, single-domain antibodies, that lack the presence of light chains. These are typically derived from members of the Camelidae family that include llamas and camels. Their variable region (VHH) is the smallest antigen-binding fragment found in a natural antibody, and nanobodies are the smallest (≈15 kD) naturally occuring immunoglobins. Further, nanobodies are stable, and can bind antigens with high affinity. Experimentally, nanobodies have been applied in WB, IF, and IP. In IP/WB applications, nanobodies can avoid IgG heavy/light chain issues that occur when using regular antibodies.