ZAP70 is a non-receptor protein tyrosine kinase (part of the Syk/Zap70 family) that is involved in signaling by the T-cell antigen receptor (TCR). Ligation of the TCR/CD3 receptor in Jurkat T-cells induces phosphoprotein complexes which contain ZAP70 (1). TCR zeta chains are initially phosphorylated by p56Lck that lead to the recruitment of ZAP70 via its SH2 domain. ZAP70 in turn phosphorylates other proteins in the TCR-phosphoprotein complex. ZAP70 tyrosine kinase is tyrosine phosphorylated in Jurkat T cells and in purified peripheral T cells after MHC-I ligation. The phosphorylation of ZAP70 after MHC-I ligation is dependent on TCR/CD3 surface expression. One of the natural substrates for ZAP70 is the zeta-chain dimer of the TCR/CD3 compl
Size
5 μg
Concentration
5μg/50 μl
Applications
RUO
Other Names
ZAP70, zeta-chain (TCR) associated protein kinase 70kDa, Syk-related tyrosine kinase