The Eagle Biosciences Glutamate ELISA assay kit employs the competitive quantitative sandwich enzyme immunoassay technique. An antigen has been pre-coated onto a microtiter plate. After extraction and derivatization, controls, standards or samples are pipetted into the wells together with specific Glutamate antiserum. The standards, controls and samples and the solid phase bound analyte antigen compete for a fixed number of antiserum binding sites. When the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. Following a washing to remove unbound substances, anti-rabbit IgG conjugated to Peroxidase is added to each well and incubated. After washing away any unbound antibody conjugate, a substrate solution (TMB) is added to the wells and color develops in inverse-proportion to the amount of glutamate present in the samples. The color development is stopped by the addition of STOP solution and the intensity of the color is measured at a wavelength of 450nm ±2nm. The concentration of glutamate in the sample is then determined by comparing the O.D of samples to the standard curve. Sample Types: serum, EDTA-plasma, urine. Range: 0.6-60 μg/ml. Sensitivity: 0.3 μg/ml. Time: 3 hours ?