Protein G purification is a process for isolating the IgG type of immunoglobulin protein from a complex mixture. The starting material is usually but not limited to polyclonal antisera, tissue culture supernatant, or ascites fluid. Typically protein G purification is performed when IgG enrichment is desired, and is often used to purify IgG prior to conjugation to a reporter molecule. Protein G may offer improved purifcation compared to Protein A for certain immunoglobulin classes and for certain host organisms.The IgG is bound to an immobilized Protein G matrix, non-IgG impurities are wased away, lastly the IgG fraction is eluted and recovered. Rockland's Protein G purification process is ideal to recover a highly active IgG fraction from complex starting material.
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Other Names
Protein G chromatography, IgG purification, IgG fractionation, antibody purification, purification