With the advancement in mass spectrometry technology, protein and peptide identification is no more tedious and time consuming. To help shorten the time duration of protein identification and enhance the accuracy of protein sequencing, Creative Proteomics has been long focused on optimizing our mass spectrometry technology platform. We have developed a comprehensive protein identification pipeline, which starts from sample preparation, protein separation and purification, followed by mass analysis, and finally to systematic bioinformatics analysis.