Annexin V (Biotin) Apoptosis Detection BioAssay? Kit
Description
Sample Type: Living cells (suspension and adherant) Intended Use: For use in detecting the early/middle stages of apoptosis and to differentiate apoptosis from necrosis. Specificity: Recognizes Annexin V. Species Crossreactivity: mammalian Test Principle: The Annexin V-Biotin Apoptosis Detection BioAssay? Kit is based on the observation that soon after initiating apoptosis, most cell types translocate the membrane phospholipid phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can easily bind to Biotin-conjugated Annexin V, a protein that has a strong natural affinity for PS. Annexin V-Biotin can be detected in conjunction with conventional dye-staining using any streptavidine- or avidin-dye reagents, such as (strept)avidine-fluorescein, -peroxidase, -alkaline phosphatase (AP), and -beta-gal, etc. Results can be analyzed by flow cytometry or fluorescence microscopy. Kit Components: Annexin V-Biotin, 1x125ul ?1X Bin