Kallikrein 1 (KLK1) BioAssayTM ELISA Kit (Rat) is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of KLK1 in rat serum, plasma, tissue homogenates and other biological fluids. Detection Range: 123.5-10,000pg/ml Sensitivity: 48.2pg/ml Intra-Assay CV: <10% Inter-Assay CV: <12% Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific for KLK1 has been pre-coated onto a microplate. A competitive inhibition reaction occurs between biotin-labeled KLK1 and unlabeled KLK1 (standards or samples) for limited binding sites on the pre-coated antibody specific for KLK1. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is inversely proportional to the concentration of KLK1 in the sample. After addition of the substrate solution, the intensity of c