Purified by immunoaffinity chromatography to ≥ 95% purity as determined by SDS-PAGE, reduced. Gene cloned from T-cell-tropic HIV-1, MN strain and the full length recombinant envelope gp120 glycoprotein produced in the Baculovirus Expression System. This protein binds to murine monoclonal antibodies of defined epitope specificity and human serum polyclonal antibodies in ELISA and Western Blot. Biological Activity: This protein binds to human T-cell receptor CD4 in ELISA and Western ELISA as determined by CD4/gp120/Anti-gp120 mAb-peroxidase capture ELISA. This protein activates human T-Lymphocytes (CD4+, CD4-), in vitro, as measured by RNA synthesis during G0 to G1 transition phase of antigen-binding competent Cells. Applications: Suitable for use in ELISA and Western Blot.