Purity: > 95% by SDS - PAGE Format: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 20% glycerol, 0.2mM PMSF . ADP-ribosylation factor 6, also known as ARF6, is a member of the ADP ribosylation factor family of GTP-binding proteins. It is localized to the plasma membrane, and regulates vesicular trafficking, remodelling of membrane lipids, and signaling pathways that lead to actin remodeling. Additionally, ARF6 play a central role in maintenance of organelle integrity, assembly of coat proteins and activation of phospholipase D. Recombinant human ARF6 protein , fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.