The Human Insulin (INS) ELISA Kit is a competitive inhibition immunoassay for the in vitro quantitative measurement of INS in human serum, plasma, cell culture supernatant and other biological fluids. Detection Range: 61.7-5000pg/ml Sensitivity: <24.6pg/ml Precision: Intra-Assay CV: <10% Inter-Assay CV: <12% Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific for INS has been pre-coated onto a microplate. A competitive inhibition reaction occurs between biotin-labeled INS and unlabeled INS (standards or samples) with the pre-coated antibody specific for INS. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is inversely proportional to the concentration of INS in the sample. After addition of the substrate solution, the intensity of color developed is inversely proportional to