ELISA, Western Blot. This purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 19.9 kDa in size corresponding to the mature human IL-29 protein by western blotting in appropriate cell lysate or extract.
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Description
Anti-IL-29 is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for human IL-29 protein. A BLAST analysis was used to suggest cross-reactivity with IL-29 from human sources based on 100% homology with the immunizing sequence. Based on high to 100% homology, there is a chance of cross-reactivity to IL-29 from a wide variety of animals. Cross-reactivity with IL-29 from other sources has not been determined.
Immunogen
This IgG fraction antibody was prepared from rabbit antiserum after repeated immunizations with mature length recombinant human IL-29 protein produced in E.coli.