ELISA, Western Blot. This product has been assayed against 1.0 ug of Glucose Dehydrogenase [Bacillus] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:7,500 to 1:32,000 of the reconstitution concentration is suggested for this product.
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Description
This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Glucose Dehydrogenase [Bacillus]. Cross reactivity against Glucose Dehydrogenase from other sources is unknown.