ELISA, Immunoprecipitation, Western Blot. Anti-Glutamine Synthetase antibody has been assayed against 1.0 μg of Glutamine Synthetase [Microbial] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:16,000 of the reconstitution concentration is suggested for this product. It is suitable for Immunohistochemistry also. Specific conditions for reactivity should be optimized by the end user.
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Description
Anti-Glutamine Synthetase antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Glutamine Synthetase [Microbial]. Cross reactivity against Glutamine Synthetase from other sources is unknown.
Immunogen
Glutamine Synthetase [native from brevibacterium species]