ELISA, Immunohistochemistry, Western Blot. This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 19 kDa in size corresponding to GPx4 protein by western blotting in the appropriate cell lysate or extract.
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Description
This affinity purified antibody is directed against mouse GPx4 protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest cross-reactivity with GPx4 protein from mouse, rat and guinea pig based on 100% homology with the immunizing sequence. Cross-reactivity with human GPx4 is also predicted based on a 94% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids near the carboxyl terminus of mouse GPx4 protein.