ELISA, Immunohistochemistry, IF Microscopy, Western Blot. This antibody has been tested for use in ELISA, immunohistochemistry, western blotting and immunoprecipitation. Reactivity in other immunoassays is unknown. Serum starved 293T whole cell lysate is suitable for use as a positive control. Anti-GSK 3B shows a strong signal to GSK 3B in westerns at the estimated molecular weight of 47 kD. This antibody may show very weak reactivity against GSK 3A.
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Description
This affinity purified antibody is directed against the phosphorylated form of human GSK3B at the pS9 residue. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide. This phospho specific polyclonal antibody reacts with phosphorylated pS9 of human GSK3B. Reactivity with non-phosphorylated human GSK3B is minimal. A BLAST analysis was used to suggest reactivity with this protein from human, rat, frog, chicken, dog and zebrafish based on 100% homology for the immunogen sequence. Cross reactivity with GSK3B homologues from other sources has not been determined.
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the aa 4-12 of human GSK3 beta.