ELISA, Immunohistochemistry, Western Blot. Suitable for ELISA, western blotting, fluorescent assays, immunoprecipitation, immunodiffusion, conjugation to magnetic particles (beads) and most other immunological methods requiring lot-to-lot consistency, high titer and specificity. Although not specifically tested, this reagent should also be useful for FACS and other fluorescent immunoassays. R-Phycoerythrin (240 kDa) is a labile molecule that may dissociate into components upon exposure to reducing or denaturing agents. Reaction with low molecular fragments is typically noted by western blot.
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Description
This product was prepared from monospecific antiserum by immunoaffinity chromatography using a R-Phycoerythrin coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and R-Phycoerythrin conjugated IgG. This antibody will cross react with B-Phycoerythrin. Reactivity with other phycobiliproteins is unknown.
Immunogen
This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with highly purified R-Phycoerythrin from the seaweed gracilaria.
Other Names
goat anti R-Phycoerythrin Antibody, goat anti-R-Phycoerythrin Antibody, goat anti Phycoerythrin