ELISA, Western Blot. This antibody reacts with human and mouse c-myc protein, which is a nuclear protein with a molecular weight of approximately 66 kDa. This antiserum detects c-myc by immunoblotting assays in both overexpressed and endogenous systems. Reactivity in other immunoassays has not yet been fully determined although perinuclear and cytoplasmic staining has been reported. The recommended positive controls are SK-BR-3 cells (adenocarcinoma, breast, malignant pleural effusion, human).
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Description
This antiserum is directed against human c-myc and is useful in determining its presence in various assays. Anti-c-myc is highly specific for c-myc and shows no cross-reaction with N-myc or L-myc proteins.
Immunogen
This whole rabbit serum was prepared by repeated immunizations with c-myc peptide corresponding to amino acids KHKLEQLRNSGA which map to the last 12 amino acids of the mouse
Other Names
rabbit anti-c-Myc Antibody, Myc proto-oncogene protein antibody, Proto-oncogene c-Myc antibody, Transcription factor p64 antibody, Class E basic helix-loop-helix protein 39 antibody