ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot. Anti-Collagen antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, immunoprecipitation, native (non-denaturing, non-dissociating) PAGE, immunohistochemistry, and western blotting for highly sensitive qualitative analysis.
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Description
Collagen III Antibody has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities. Typically negligible cross-reactivity against other types of collagens was detected by ELISA against purified standards. Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type III collagens and has negligible cross-reactivity with Type I, II, IV, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
Immunogen
Collagen Type III from human and bovine placenta
Other Names
rabbit anti-Collagen Type III antibody, Collagen type III alpha 1 antibody, Collagen type III alpha antibody, EDS4A antibody, Ehlers Danlos syndrome type IV, autosomal dominant antibody, Fetal collagen antibody, COL3A1, Collagen alpha-1 (III) chain
Mak KM, Kwong AJ, Chu E, Hoo NM. (2011) Hepatic Steatosis, Fibrosis, and Cancer in Elderly Cadavers. Cancer Biology. 5 DEC 2011. DOI: 10.1002/ar.21525.Santanu Chakraborty, Elaine E. Wirrig, Robert B. Hinton, Walter H. Merrill, Douglas B. Spicer, Katherine E. Yutzey. (2010) Twist1 promotes heart valve cell proliferation and extracellular matrix gene expression during development in vivo and is expressed in human diseased aortic valves. https://doi.org/10.1016/j.ydbio.2010.08.021.