ELISA, Immunohistochemistry, Western Blot. This Anti-Mouse IgG subclass pan reactive Secondary Antibody is is designed for Western Blotting, ELISA and Immunohistochemistry. HRP conjugated secondary antibodies can also be used for a variety of other applications such as Assay Development. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment.
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Description
Anti-Mouse IgG subclass pan reactive Secondary Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. This product shows balanced reactivity to Mouse IgG1, IgG2a, IgG2b and IgG3 proteins and is suitable to screen IgG class hybridoma clones. Minimal cross reactivity is observed against other Mouse immunoglobulin classes or light chain proteins.