ELISA, Immunohistochemistry, Western Blot. This product has been assayed against 1.0 ug of Guinea Pig IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:28,000 to 1:114,000 of the reconstitution concentration is suggested for this product.
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Description
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Guinea Pig IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Guinea Pig IgG, Guinea Pig IgG F(ab')2 and Guinea Pig Serum. No reaction was observed against Guinea Pig IgG F(c).
Immunogen
Guinea Pig IgG F(ab')2 fragment
Other Names
Goat Anti-Guinea Pig IgG F(ab')2 Biotin Conjugated Antibody, Goat Anti Guinea Pig IgG F(ab')2 Antibody Biotin Conjugated , Goat Anti Guinea Pig IgG Fab2 Fragment Biotin Conjugated Antibody