ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot. Anti-DDB1 antibody reacts with human and mouse DDB1 by western blot and immunoprecipitation. The antibody immunoprecipitates in vitro translated protein and protein from cell lysates (using HeLa, NIH-3T3, and others). Coimmunoprecipitation of related proteins has not been tested. A 127.0 kDa band corresponding to human DDB1 is detected. Most cell lines expressing DDB1 can be used as a positive control. Researchers should determine optimal titers for other applications.
Reviews / Ratings
If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY.
Description
Anti-DDB1 is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. This product reacts with human and mouse DDB1. Cross reactivity with DDB1 from other sources is not known.
Immunogen
DDB1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 198-213 of Human DDB1 (internal) coupled to KLH.
Other Names
rabbit anti-Anti-p127-DDB1 antibody, Damage specific DNA binding protein 1 antibody, DDB 1 antibody, DDB p127 subunit antibody, DDBa antibody, DNA damage binding protein 1 antibody, UV damaged DNA binding factor antibody
Shiyanov P, Nag A, Raychaudhuri P. (1999) Cullin 4A associates with the UV-damaged DNA-binding protein DDB. J Biol Chem. 274(50):35309-12.Shiyanov P, Hayes SA, Donepudi M, Nichols AF, Linn S, Slagle BL, Raychaudhuri P. (1999) The naturally occurring mutants of DDB are impaired in stimulating nuclear import of the p125 subunit and E2F1-activated transcription. Mol Cell Biol. (7):4935-43.