ELISA, Immunohistochemistry, Western Blot. This protein A purified antibody has been tested for use in ELISA, immunohistochemistry and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 67 kDa in size corresponding to NRF1 by western blotting in the appropriate cell lysate or extract. Splice variants exist for this protein that may result in the detection of lower molecular weight bands.
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Description
This protein A purified antibody is directed against mouse NRF1. The product was purified from monospecific antiserum by protein A affinity purification. BLAST analysis was used to suggest reactivity with this protein from mouse, human, chimpanzee, dog, rat, chicken, frog and zebrafish based on very high sequence homology with the immunogen sequence. Cross reactivity with NRF1 homologues from other sources has not been determined.
Immunogen
This protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a purified recombinant mouse NRF1 protein corresponding to aa 1- 534 of the native protein.
Other Names
rabbit anti-NRF1 antibody, rabbit anti-NRF 1 antibody, NRF-1, alpha pal antibody, alpha palindromic binding protein antibody, locus control region factor 1 antibody, NFE2 related factor 1 antibody, nuclear respiratory factor 1 antibody, transcription factor 11 antibody