ELISA, Western Blot. This affinity purified Nrf2 antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 66.9 kDa in size corresponding to Nrf-2 proteins by western blotting in the appropriate cell lysate or extract.
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Description
This affinity-purified antibody is directed against mouse Nrf2 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross reactivity with Nrf2 proteins from human, mouse, rat and bovine based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
Immunogen
Anti-NRF-2 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding near the c-term region.