IF Microscopy, Immunoprecipitation, Western Blot. This antibody has been tested for use in immunofluorescence microscopy, immunoelectron microscopy, immunoprecipitation and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 211 kDa in size corresponding to SARS-CoV nsp3 by western blotting in the appropriate cell lysate or extract. For immunofluorescence microscopy, Vero-E6 cells, grown on glass slides, were infected with SARS-CoV-Fr1 strain for 1 h at 37°C. Infection occurred in PBS/DEAE/2%FCS followed by exchange to EMEM/25mMHEPES/2%FCS. Cells were fixed with PBS/3%PFA. After washing fixed cells, antibody incubation was performed in PBS/5%FCS for 30 min.
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Description
This antibody is directed against SARS-Coronavirus nsp3 protein. The product is neat antiserum. Cross reactivity with homologues from other sources has not been determined.
Immunogen
This whole rabbit serum was produced by repeated immunizations with 3 E.coli derived proteins representing different parts of the SARS-CoV Nsp3 protein: aa 834-943 of polyprotein 1a (ADRP domain), aa 1208-1557 of polyprotein 1a (SARS-Unique Domain) and aa 1558-2040 of polyprotein 1a (Papain-like proteinase domain).
Other Names
rabbit anti-Sars-Cov Nonstructural Protein 3 Antibody, Shep1 antibody, Cas/HEF1 associated signal transducer antibody, CHAT antibody, FLJ39664 antibody, Novel SH2 containing protein 3 antibody