human, mouse, rat, bovine, guinea pig, hamster, monkey, rabbit
Isotype
IgG1
Format
Protein G purified
Size
100 μg
Concentration
1mg/mL
Applications
ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot. Anti-HSF1 Antibody is suitable for WB, IP, IF microscopy, ELISA, and Gel mobility shift assay. Expect a band approximately ~85kDa protein in unstressed cell lysates, and an ~95kDa protein in heat shocked cell lysates, corresponding to the molecular mass of inactive and active forms of HSF1 on SDS PAGE immunoblots. Specific conditions for reactivity should be optimized by the end user.
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Description
Anti-HSF1 Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with HSF1 from Human, Mouse, Rat, Bovine, Guinea Pig, Hamster, Monkey, and Rabbit based on 100% homology with the immunizing sequence. Cross-reactivity with HSF1 from other sources has not been determined. Heat Shock research.
Immunogen
HSF1 Antibody was produced in rat by repeated immunizations with purified recombinant HSF1 protein.