ELISA, Immunohistochemistry, Western Blot. Anti-Guinea Pig (H&L) biotin conjugated antibody generated in goat detects specifically guinea pig IgG (H&L). This secondary biotin conjugated antibody anti-Guinea Pig is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays. Antibody anti guinea pig biotin conjugated has been assayed against 1.0 ug of Guinea Pig IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:50,000 of the reconstitution concentration is suggested for this product.
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Description
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Guinea Pig IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Guinea Pig IgG and Guinea Pig Serum.
Immunogen
Anti-Guinea Pig IgG (H&L) was produced by repeated immunization with guinea pig whole molecule in goat.