ELISA, Western Blot. This affinity purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band ~ 54 kDa in size corresponding to CaM Kinase II by western blotting in the appropriate cell lysate or extract.
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Description
This affinity purified antibody is directed against human CaM Kinase II protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity with this protein from human, mouse, rat, dog and orangutan based on 100% homology for the immunogen sequence. Cross reactivity with CaM Kinase II protein from zebrafish, chiken, pig, rabbit and frog may occur as this sequence only varies by one amino acid residue (94% homology). Cross reactivity with CaM Kinase II homologues from other sources has not been determined.
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 6-23 of Human CaM Kinase II protein.
Other Names
rabbit anti-CaM Kinase II Antibody, Calcium/calmodulin dependent protein kinase II alpha antibody, CaM kinase II alpha antibody, CaM kinase II α chain antibody, CaM kinase II alpha subunit antibody, CAMK2A, CAMKA