ELISA, Western Blot. This antiserum has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band ~ 52 kDa in size corresponding to CaM Kinase IV by western blotting in the appropriate cell lysate or extract.
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Description
This antiserum is directed against human CaM Kinase IV protein. The product was delipidated, defibrinated followed by buffering and clarification. A BLAST analysis was used to suggest reactivity with this protein from human, mouse, rat, dog, bovine and chimpanzee based on 100% homology for the immunogen sequence. Cross reactivity with CaM Kinase IV protein from zebrafish, chicken and frog may occur as this sequence only varies by one or two amino acid residues (~89% homology). Cross reactivity with CaM Kinase IV homologues from other sources has not been determined.
Immunogen
This antiserum was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 305-323 of Human CaM Kinase IV protein.
Other Names
rabbit anti-CaM Kinase IV Antibody, CAM kinase 4 antibody, CAM kinase GR antibody, CAM kinase IV antibody, CaMK4 antibody, CaMKGR antibody, Brain Ca(2+) calmodulin dependent protein kinase type IV antibody, Calcium / calmodulin dependent protein kinase type 4 catalytic chain antibody
Gene, Accession #
CaMK IV, Gene ID: 25050, NCBI: 60392502, UniProt: P13234