Total RNA was prepared from cell line WM3623. WM3623 is a metastatic human melanoma cell line. This cell line was established from a right lymph node metastasis site in a 48-year-old male with level V metastatic malignant melanoma. This cell line contains a Q61K mutation at position 61 in the N-RAS gene which causes increased signaling via the extracellular signal-regulated MAPK/ERK kinase pathways to enhance proliferation. WM3623 cells produce xenograft tumors when injected into immunocompromised mice.
Size
10 μg
Concentration
200 ng / μl
Applications
Purified RNA is suitable for a number of molecular biology applications including but not limited to next generation sequencing and expression library construction, PCR, real-time PCR, microarray analysis, and transfection experiements.
Other Names
Human Melanoma, patient derived tumor, tumor models, skin cancer, xenograft