Total RNA was prepared from cell line WM2044. WM2044 is a metastatic human melanoma cell line that displays mesenchymal morphology. These cells require insulin for growth in culture. This cell line features the specific V600E (Val600Glu) mutation at codon 600 in the BRAF gene. This mutation causes constitutively active kinase activity and activation of MEK and ERK signaling pathway. This cell line also expresses PTEN loss of function including hemizygous deletion, and is wild type for N-RAS, c-KIT, and CDK4. WM2044 cells produce xenograft tumors when injected into immunocompromised mice.
Size
10 μg
Concentration
200 ng / μl
Applications
Purified RNA is suitable for a number of molecular biology applications including but not limited to next generation sequencing and expression library construction, PCR, real-time PCR, microarray analysis, and transfection experiements.
Other Names
Human Melanoma, patient derived tumor, tumor models, skin cancer, xenograft