Sample Type: Serum, Plasma, Biological Fluids Intended Use: This BioAssay? kit is a competitive ELISA for in vitro quantitative measurement of BNP in rat serum, plasma and other biological fluids Sensitivity: 18.75pg/mL Range: 31.25-2000pg/mL Specificity: Rat Test Principle: This BioAssay? ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with rat BNP (Brain Natriuretic Peptide). During the reaction, rat BNP (Brain Natriuretic Peptide) in the sample or standard competes with a fixed amount of rat BNP (Brain Natriuretic Peptide) on the solid phase supporter for sites on the Biotinylated Detection Ab specific to rat BNP (Brain Natriuretic Peptide). Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by adding