Purity ≥ 95% as determined by RP-HPLC and SDS-PAGE. Protein phosphatase 2C alpha (PP2Ca) is a Mn2+- or Mg2+-dependent protein serine/threonine phosphatase that is essential for regulating cellular stress response in eukaryotes. The protein coding region of PP2Ca (aa1-382) was cloned into an E. coli expression vector (BamHI/HindIII site). PP2Ca was overexpressed in E. coli as a soluble His-tag fusion protein, and it was purified by conventional column chromatographic techniques. Recombinant human PP2Ca is a single, non-glycosylated polypeptide chain containing 382aa (containing His tag, T7 gene 10 leader, XpressTM Epitope). AA Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWILMGAF LDKPKMEKHN AQGQGNGLRY GLSSMQGWRV EMEDAHTAVI GLPSGLESWS FFAVYDGHAG SQVAKYCCEH LLDHITNNQD FKGSAGAPSV ENVKNGIRTG FLEIDEHMRV MSEKKHGADR SGSTAVGVLI SPQHTYFINC GDSRGLLCRN RKVHFFTQDH KPSNPLEKER IQNAGGSVMI QRVNGSLAVS RALGDFDYKC VHGKGPTEQL VSPEPEVHDI ERSEEDDQFI ILACDGIWDV MGNEELCDFV RSRLEVTDDL EKVCNEVVDT CLYKGSRDNM SVIL
Size
10ug, 50ug
Concentration
n/a
Applications
n/a
Other Names
Protein Phosphatase 2C alpha, Recombinant, Human (PP2Ca, PPM1A, Protein Phosphatase, Mg2+/Mn2+ Dependent, 1A)