An enzyme immunoassay for the quantitative measurement of total 5Alpha-dihydrotestosterone (DHT) in serum or plasma (heparin- or citrate- plasma). This product is for Research Use Only; International customers contact your representative to receive information about the CE marked version. The DRG DHT-optimized ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA), based on the principle of competitive binding. The microtiter wells are coated with a polyclonal antibody directed towards an antigenic site of the DHT molecule. Endogenous DHT of a patient sample competes with a DHT-horseradish peroxidase conjugate for binding to the coated antibody. After incubation the unbound conjugate is washed off. The amount of bound peroxidase conjugate is inversely proportional to the concentration of DHT in the sample. After addition of the substrate solution, the intensity of colour developed is inversely proportional to the concentration of DHT in the patient sample.