The competitive Homoarginine ELISA Assay Kit uses the microtiter plate format. Homoarginine is bound to the solid phase of the microtiter plate. Homoarginine in the samples is acylated and competes with solid phase bound Homoarginine for a fixed number of rabbit anti-Homoarginine antiserum binding sites. When the system is in equilibrium, free Antigens and free Antigens-antiserum complexes are removed by washing. The antibody bound to the solid phase Homoarginine is detected by anti-rabbit/peroxidase. The substrate TMB / peroxidase reaction is monitored at 450 nm. The amount of antibody bound to the solid phase Homoarginine is inversely proportional to the Homoarginine concentration of the sample. Sample Types: serum, plasma, cell culture. Range: 0.3 - 7.0 μmol/L. Sensitivity: 0.05 μmol/L. Time: 2.5 hours ? Homoarginine is a non-essential cationic amino acid, which is formed from lysine. In vitro and in vivo, homoarginine shows characteristics similar to arginine. Epidemiological investigations in two large independent cohorts, namely the German diabetes dialysis (4D) study and the Ludwigshafen Risk and Cardiovascular Health (LURIC) study have identified homoarginine as useful predictor of cardiovascular events and mortality.