Mouse CD300LB is a member of the CD300 family of molecules that have regulatory function in the immune system. The human and mouse families have seven and nine members respectively. The human genes are localized on chromosome 17 and the mouse in the syntenic region on chromosome 11. The CD300 receptors are type I attached membrane proteins that contain one IgV-like extracellular domain having two disulfide bonds. In addition, mouse CD300LB possesses a positive amino acid (Lys) in its transmembrane domain and a short cytoplasmic tail that lacks a signaling motif. Mouse CD300LB associates with its adaptor molecules DAP10 (DNAX-activating protein-10) and DAP12 through the positive charge amino acid (Lys) in the transmembrane domain. Studies with transduced bone marrow-derived mast cells have shown that DAP12 plays a primary role in functions of mouse CD300LB, and DAP12 and DAP10 in the human receptor. CD300LB is expressed in myeloid cells and its transduction in bone marrow–derived mast cells induces cell survival, degranulation, cytokine production, and adhesion to the extracellular matrix. Mouse CD300LB is associated with LPS-induced sepsis in mice and a soluble form of CD300LB amplifies LPS-induced lethal inflammation in sepsis. Neutrophils release a soluble form of CD300LB through a proteolytic process with possible participation of MMPs and/or ADAM-TS family members. Raw 264.7 cells, but not macrophages, also release soluble CD300LB and this release is induced by LPS and it is dose dependent. LPS induces the interaction of CD300LB to TLR4 and drives lethal cytokine responses that boost septic shock.
Size
100 μg
Concentration
n/a
Applications
BA
Other Names
CD300 antigen like family member B, Leukocyte mono-Ig-like receptor 5 (LMIR5), CMRF35-like molecule 7 (CLM-7, Clm7), Immune receptor expressed on myeloid cells 3 (IREM-3), Gm253