Plasminogen Activator Inhibitor 1 (PAI-1) is a glycoprotein found in plasma that is implicated in the processes of angiogenesis, hemostasis, tumor metastasis, fibrionlysis, ovulation, embryonic development and inflammation (1). The elevation of PAI-1 has shown to correlate with the prognosis of many cancers including breast cancer (2,3,4). PAI-1 is activated by the glycoprotein vitronectin. Vitronectin binds to PAI-1 and modulates fibrinolysis and cell migration (5). This binding has proven to be an important inhibitor of the activated form of protein C (APC) (6). Kit Components: P4256-32A: PAI-1 Activity Standard (0u/ml): 1x1ml P4256-32B: PAI-1 Activity Standard (150-350u/ml): 1x1ml P4256-32C: TMB Substrate: 1x10ml P4256-32D: PAI-1 Mab: 1x1 vial P4256-32E: Secondary Antibody (HRP): 1x1 vial P4256-32F: Wash Buffer (10X): 1x50ml P4256-32G: ELISA Buffer: 1x10ml P4256-32H: Primary Antibody Buffer: 1x10ml P4256-32J: Secondary Antibody Buffer: 1x15ml P4256-32K: Microtiter Plate: 1x96 wells