Plasminogen Activator Inhibitor Type 1, Human, Recombinant (PAI 1, PAI-1)
Description
Purity ~98% (SDS PAGE, HPLC). PAI-1 (plasminogen activator inhibitor-1) is a serine protease inhibitor and member of the serpin family, which acts as the primary inhibitor of tissue plasminogen activator (tPA) and urokinase plasminogen activator (uPA), and hence fibrinolysis. Increased levels of PAI-1 are associated with diabetes, cardiovascular disease and an increased occurrence of thrombosis in obesity, and can be induced by obesity related factors including insulin, glucose and VLDL (very-low-density lipoprotein). Source: Recombinant corresponding to human PAI-1, expressed in E. coli. Molecular Weight: ~42.7kD (379aa sequence) Activity: Determined by its inhibitory effect against single chain tPA induced cleavage of a chromogenic substrate in Imidazole Buffer at 37oC. Half maximal inhibition against 1.0ug/ml of single chain tPA was obtained at a concentration of 2.0ug/ml. Endotoxin: ≤1EU/ug Applications: Suitable for use in Functional Assays.