eNOS KO Mouse Artery Smooth Muscle Cells from Creative Bioarray are isolated from artery of pathogen-free laboratory mice. eNOS Knockout Mouse Artery Smooth Muscle Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in Creative Bioarray Culture Complete Growth Medium generally for 3-7 days. Prior to shipping, cells at passage 1 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen. The cells are characterized by immunofluorescent method with antibodies to α-smooth muscle actin. eNOS Knockout Mouse Artery Smooth Muscle Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded on a multiwell culture plate ready for experiments under the cell culture conditions specified by Creative Bioarray (Cells may be expanded for 2 or 3 passages at a split ratio of 1:2 under the cell culture conditions specified by Creative Bioarray). Repeated freezing and thawing of cells is not recommended.