NCI-H69 VCR/R has been derived from the parent small cell lung cancer line NCI-H69. It is recommended to culture cells without drug upon resuscitation; add vincristine once growth is fully established. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.